Rescuing the

American Chestnut

Our Mission

is to return the iconic American chestnut to its native range.

Our Vision

is a robust eastern forest restored to its splendor.

Make a difference this holiday season

Your support allows TACF to continue working from the ground up toward restoration of the American chestnut. Please help us reach our goal.

Chestnut Chat Series

Darling 58, Transgenics, and Science Strategy Updates

Join us December 15 from 11:30AM – 1:00PM (EPT), for the next LIVE Chestnut Chat. TACF will share the latest updates about the status of the Darling transgenic American chestnut tree and new information since our last D58 Chestnut Chat in September. Panelists will present data, analyses, and frequently asked questions, then give participants an opportunity to ask their own questions.

Meadowview Research Farms
TACF 40th Anniversary logo

Join the celebration!

The American Chestnut Foundation is celebrating its 40th anniversary! We invite you to join us at one of the many chapter festivities this year. Non-members are welcome to attend.

Map of TACF 40th Anniversary celebrations

Science Strategies

Breeding, Biotechnology, and Biocontrol
United for Restoration

The American Chestnut Foundation takes a holistic approach toward chestnut restoration, utilizing a three-pronged research strategy known as 3BUR (Breeding, Biotechnology, and Biocontrol United for Restoration). These research tracks are meant to be integrated through collaborations that are mutually beneficial, so we can explore all avenues to reach the common goal of saving and restoring this species as quickly as possible.

Support Our Research

How to identify an American chestnut tree

Learn how to identify American chestnuts and send us a sample for identification.

Support The Restoration

How to receive American chestnut seeds & seedlings

Learn how to get American chestnut seeds and seedlings from TACF.

Latest News

Press Release: TACF Discontinues Development of Darling 58

Press Release: TACF Discontinues Development of Darling 58

TACF Discontinues Development of Darling 58Asheville, NC, December 8, 2023 The American Chestnut Foundation (TACF) announced today that it will discontinue its development of the Darling 58 American chestnut due to significant performance limitations that, from TACF’s...

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WV Chapter President Mark Double Retires

WV Chapter President Mark Double Retires

By WV Board Member Lewis CookOver a brisk weekend in early October, during the Annual West Virginia Chestnut Festival in Rowlesburg Mark Double, WV-TACF Chapter President, retired from his duties. Chapter Board member Lewis A. Cook shared these kind words about his...

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Join us December 15 from 11:30AM – 1:00PM (EPT), for TACF’s next LIVE Chestnut Chat. TACF will share the latest updates about the status of the Darling transgenic American chestnut tree and new information since our last D58 Chestnut Chat in September. Panelists will present data, analyses, and frequently asked questions, then give participants an opportunity to ask their own questions. Visit the link in the comments below to learn more and sign up. ... See MoreSee Less

Join us December 15 from 11:30AM – 1:00PM (EPT), for TACF’s next LIVE Chestnut Chat. TACF will share the latest updates about the status of the Darling transgenic American chestnut tree and new information since our last D58 Chestnut Chat in September. Panelists will present data, analyses, and frequently asked questions, then give participants an opportunity to ask their own questions. Visit the link in the comments below to learn more and sign up.Image attachmentImage attachment

6 CommentsComment on Facebook

For those wondering, SUNY-ESF continues this work and is still refining it. To publicly pull their funding like this is disgusting. The level of unprofessional behavior in today’s release is appalling! I’m moving my financial support to ESF.

I was just reading the the Darling 58 research will be discontinued.

Will all the funds that NY had to surrender to tacf go back to NY ?

EDIT As a member. I was pretty confused by what seemed to be a sudden abandonment of 1 of three 3 burrs in the plan, which was 3 burr. That's not the case, but one needs to dig DEEPLY into TACF's page to get an explanation as to why the decision is being made. See my other comment above. I'm not happy with how this was handled.

Deep, and I mean DEEP within TACF's "Darling58" performance page is an indication that they were not testing D58, but were testing a different line the whole time without knowing it: "In November 2023, through molecular analyses performed by partners at the University of New England and University of Maine, TACF learned that the OxO gene of all Darling 58 trees was on a different chromosome than expected (chromosome 4 instead of chromosome 7). Upon further and additional independent investigation, scientists confirmed that the trees they had been researching were in fact descendants of a different event in the Darling line in which the OxO gene had been inserted into a coding region, causing a deletion in a known gene. That research has also indicated that the homozygous state (when an individual plant inherits the OxO gene from both parents, which occurs in 25% of offspring) is lethal, and that a majority of homozygous offspring die in the embryonic stage." EDIT link to page :https://tacf.org/darling-58/ EDIT - The page mentions that "other better trees" are being focused on, reading even farther, it looks like TACF would like to focus on testing SUNY's "DarWIN" going forward, which has the oxo gene as wound inducible. Considering they would have to start testing all over again for D58, I guess it makes sense to just start with DarWIN. OPINION: It would have been better to say "whoops" and acknowledge that they were testing the wrong thing as the FIRST point in the conversation, then point to reduced growth of D58 in some trials possibly due to constant expression of oxo, and finally state that they were going forward with some of SUNY's most advanced trees since they might as well. The presentation should have come from a place of acknowledging how stuff was screwed up and continuing support of other transgene lines. Instead it comes across as abandonment. You have to go very VERY deep and make a little inference to get a solid grasp on what the situation is, and you shouldn't have to.

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The American Chestnut Foundation (TACF) announced today that it will discontinue its development of the Darling 58 American chestnut due to significant performance limitations that, from TACF’s perspective, make it unsuitable as a restoration tree. Likewise, TACF is also withdrawing its support for several pending regulatory petitions that would authorize distribution of transgenic Darling trees outside permitted research plots.

To read the entire press release, visit the link in the comments. We'll be discussing this topic at length during the December 15 Chestnut Chat.
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The American Chestnut Foundation (TACF) announced today that it will discontinue its development of the Darling 58 American chestnut due to significant performance limitations that, from TACF’s perspective, make it unsuitable as a restoration tree. Likewise, TACF is also withdrawing its support for several pending regulatory petitions that would authorize distribution of transgenic Darling trees outside permitted research plots.

To read the entire press release, visit the link in the comments. Well be discussing this topic at length during the December 15 Chestnut Chat.

31 CommentsComment on Facebook

For much more information about D58 and many frequently asked questions, please visit tacf.org/darling-58/

**EDIT TO ADD: SUNY announced they will continue to persue the Darling efforts without TACF. I know where my support will be going.** Will SUNY persue? The Darling 58 is 100% better than 100 years of failed hybridization.

If I understand the press release correctly, TACF is withdrawing support from the Darling 58 transgenic chestnut because they were erroneously given misidentified material. Why not get the error sorted and redo the trials?

Wow, this is so disappointing and must be a profoundly sad event for the long-time researchers. Let's hope one of the new, improved varieties can be brought forward for regulatory approval within my lifetime.

Wow, big news

I am so sorry to hear this news. I am very curious for more info on the pollination. I look forward to the December chat Sending love to all

I know of a large mature chestnut tree in Louisiana. Anyone interested in looking into it? I have a dozen or so nuts from it

That's disappointing but the TACF should be commended for its openness and commitment to producing quality blight resistant trees. Im sure the dedicated folks at TACF and SUNY will continue to pursue the goal of wild chestnut restoration both through transgenics and hybridization. Maybe the Darling 58 could be improved with a few generations of selected breeding while simultaneously developing new transgenic lines? I'll leave it to the experts and hope for the best.

Best news I’ve heard in a long time from ACF. I saved the email to my favorites. I’ve been saying this all along. Transgenics might be the answer but it was way way way too fast

Disappointing news. The Darling 58 route is very likely still the best avenue for successful restoration of Castanea dentata.

Is this it for transgenic chestnuts? Are there more under development?

For those wondering, SUNY-ESF continues this work and is still refining it. To publicly pull their funding like this is disgusting. The level of unprofessional behavior in this release is appalling! I’m moving my financial support to ESF’s work.

This is really sad news.

significant identity error in the propagation materials supplied to TACF. Independent confirmation now shows all pollen and trees used for this research was derived not from Darling 58, but from a different prototype, one which contains a deletion in a known gene,” says Pitt. We need to do better. I feel bad for the people who gave their money, their time and their effort only to be back to square one.

So now what? Whats plan C for Chestnut 🌰 trees 🌳

How effective is it ?

Has anyone done work on actual expression levels of OXO in Darling 58 Chestnut tissue over time? Maybe a lot of this boils down to gene silencing. I would really not be surprised if a long lived tree like American chestnut might try to shut down overly active genes sometimes as the trees age. I'm sure that gene mutation didnt help either. Homozygosity being lethal is just all kinds of bad. :/ Based on the press release I dont blame them for pulling the plug. I hope DarWin will provide better results. Very interested to see the chestnut chat in question.

I remember reading that Darling 58 was said to have noticably slow growth compared to pure american chestnut. There is another transgenic construct that is wound responsive that may be much better. Constituitive expression of oxo is probably not the way to go when youre looking at forest restoration.

Well that’s awful news.

Why can’t we just plant the Chinese chestnut? They are bigger husks n nuts 🌰 than American chestnuts. Why not introduce the Italian chestnut with an American 58..?

Disappointing!

So because D58 isn't 100% successful, it's being canned? Y'all realize nothing is 100% right? And there isn't exactly a great plan B... Seems poorly thought out

Shameful, utterly shameful

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We're hiring! TACF is seeking a Gifts and Records Specialist in our Asheville, NC office. We're accepting applications until December 15, visit the link in the comments to learn more about this position. ... See MoreSee Less

Were hiring! TACF is seeking a Gifts and Records Specialist in our Asheville, NC office. Were accepting applications until December 15, visit the link in the comments to learn more about this position.

3 CommentsComment on Facebook

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The American Chestnut Foundation is seeking scion wood from wild American chestnut trees from the southern population. Scion wood MUST come from previously verified American chestnuts. Population studies have determined that trees found in western TN and K1, GA, AL, and MS represent the southern population and are the most genetically diverse.

The objectives of this collection are to:
🌱Conserve genetic diversity of unique and underrepresented populations of American chestnut through grafting
🌱Grow these grafted plants in favorable conditions (including growth chambers) to promote flowering and ease of pollen collection
🌱Future utilization of pollen and flowers to outcross transgenic chestnut

Goals for 2024: Gather around 100 sources from the South

•Coordinate with Jamie Van Clief at jamie.vanclief@acf.org for the collection of this scion wood.
For more details- head to the link in the comments below.
... See MoreSee Less

The American Chestnut Foundation is seeking scion wood from wild American chestnut trees from the southern population. Scion wood MUST come from previously verified American chestnuts. Population studies have determined that trees found in western TN and K1, GA, AL, and MS represent the southern population and are the most genetically diverse.

The objectives of this collection are to:
🌱Conserve genetic diversity of unique and underrepresented populations of American chestnut through grafting
🌱Grow these grafted plants in favorable conditions (including growth chambers) to promote flowering and ease of pollen collection
🌱Future utilization of pollen and flowers to outcross transgenic chestnut

Goals for 2024: Gather around 100 sources from the South

•Coordinate with Jamie Van Clief at jamie.vanclief@acf.org for the collection of this scion wood.
For more details- head to the link in the comments below.

3 CommentsComment on Facebook

I have three mature chestnut trees on a property I just acquired. How does one go about getting it verified as a true American Chestnut tree. I am one hour West of Nashville TN if that qualifies.

so from Michigan no good?

Check out the Maine Chapter of TACF's booth at the Fryeburg Fair

This eagerly anticipated annual event, held each fall in the picturesque town of Fryeburg, Maine, attracts a staggering 170,000 attendees. Each year the Maine Chapter has a booth set up for people to learn about American chestnut.

This year, each person who signed up to become a new TACF member received two chestnut seedlings as part of the "Fryeburg Fair Special," and because of this, they welcomed 90 new members. This brings the total number of Chapter members to over 300 for the for the first time!
In addition to cultivating new members, their team gathered valuable insights and recorded about a dozen solid leads on the location of surviving American chestnuts in Maine.

As always, this was a successful time at the fair!

📍Maine locals- make sure to mark your calendars to attend next year!

Curious about a TACF membership? Head to the link in bio to learn more.
... See MoreSee Less

Check out the Maine Chapter of TACFs booth at the Fryeburg Fair

This eagerly anticipated annual event, held each fall in the picturesque town of Fryeburg, Maine, attracts a staggering 170,000 attendees. Each year the Maine Chapter has a booth set up for people to learn about American chestnut.

This year, each person who signed up to become a new TACF member received two chestnut seedlings as part of the Fryeburg Fair Special, and because of this, they welcomed 90 new members. This brings the total number of Chapter members to over 300 for the for the first time!
In addition to cultivating new members, their team gathered valuable insights and recorded about a dozen solid leads on the location of surviving American chestnuts in Maine.

As always, this was a successful time at the fair!

📍Maine locals- make sure to mark your calendars to attend next year!

Curious about a TACF membership? Head to the link in bio to learn more.Image attachment

1 CommentComment on Facebook

More about the ME Chapter- tacf.org/me/about-us/

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Upcoming Events

11:30AM - 1:00PM

Join us December 15 from 11:30AM – 1:00PM (EPT), for TACF’s next LIVE Chestnut Chat. TACF will share the latest updates about the status of the Darling transgenic American chestnut […]

6:00PM - 7:30PM

Chestnuts Roastingin Damascus, VA Join The American Chestnut Foundation as we get into the holiday spirit, December 16 at 6:00 PM in the Damascus Trail Center. All are invited to […]