Tennessee News

Evaluating Chestnuts in Greg’s Orchard for Blight Resistance and Phenotype

Friends,

On June 3, 2013, Dr. Hill Craddock and his UTC crew traveled to Greg Weaver’s orchard in Williamson County, TN to grade blight resistance and assess for American chestnut physical characteristics on Greg’s chestnut trees. The trees had been inoculated in June 2012 with two strains of chestnut blight (cryphonectria parasitica). Approximately 622 trees have contributed to the experiment as listed below. These include advanced backcross chestnuts (BC3’s and BC4’s), F1’s (first generation crosses American x Chinese), pure American controls, Dunstan hybrid controls (Asian type) and pure Chinese controls.

TN Rut x CH526 #116, Apr 2005
Tn Blo x GL 103 #184, Apr 2005
Rut 1 x Ft. Defiance #6, Apr 2005
Rut 1 x Lindstrom 99 #18, Apr 2005
TN Mac 1 x GL 104 #234, Apr 2006
E10 x AMY #5, Apr 2006
Dennis W #4, Apr 2006
Dunstan #20, Oct 2006
Chinese #5, Apr 2007
TN Mon 3 x OP #1, 2007
KY Met 1 x OP #1, 2007
KY Met 1 x OP #1, 2007
Whigg M x OP #1, 2007
TN Cla x Popeye #1, 2007
Istaca (American) #25, 2008

Over the years, the trees were subject to the whole range of stresses inherent in this form of plant science, including drought, pests (especially voles), a mid April hard freeze (2007), and “natural” blight infections. There was further mortality in the orchard from the experimental blight infections introduced in June 2012. The mortality from all these causes has been high with the majority of these trees having died prior to the innoculation in June 2012.

I will leave the formal reporting to Hill. By my records, nine advanced backcross hybrids (all TN Blo x GL 103) were rated as having adequate blight resistance and appropriate American chestnut physical characteristics to retain the trees in the breeding program, and to serve as the parents of the next generation. One F1 was also retained. These “blue ribbon special” trees were marked with blue ribbons tied around their trunks in the orchard, and were recorded as such on the data sheets. The TN Blo x GL 103 is known to exist only in this orchard, so retention of this germplasm is important for the TN- TACF program.

For now, all the living trees will be retained, but over the next year the lower rated trees will be removed from the orchard (think chestnut firewood).

I am grateful for the hard work and guidance from Hill and his team at UTC. Attached are some photographs from the day’s work evaluating the trees.

Greg Weaver

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The 2024 Tennessee Chapter Pollination Workshop was held on June 8 to great success, bringing together 22 enthusiastic volunteers for a day of hands-on learning at the Tennessee Tech Orchard. Established in 2005 using the direct seed planting method, the TTU backcross orchard includes a row of robust, blight resistant F1 hybrids, resulting from a 2004 cross between a naturally occurring Clay County, TN American chestnut, and the Chinese cultivar ‘Gideon.’ These F1s (originally planted as controls in the 2005 backcross orchard), in addition to their blight resistance, exhibit promising resistance to Phytophthora cinnamomi (causal agent of the phytophthora root rot disease, PRR), which has been validated through progeny tests and PRR-screening (Perkins et al., 2019). They were chosen to be the female parents this year in crosses designed to move PRR resistance into the best selections of the southern TACF chapters.

During the 2024 workshop, participants heard a short lecture from Dr. Craddock on the pollination biology of Castanea species and saw an active demonstration of emasculation and bagging of chestnut flowers. They learned about the importance of proper labelling and the labelling conventions of the TACF chapter breeders. They were then engaged directly in hand the pollination techniques used to create hybrid chestnut trees using pollen provided by colleagues in the Georgia, Carolinas, and Alabama chapters ladders and bucket lifts to access the crowns of the trees, successfully performing hundreds of pollinations.
The workshop's outcome was exceptional. The volunteers completed 27 distinct crosses. Fruit set was excellent despite the late summer drought. The burs were harvested in September yielding 2,553 hand pollinated seeds! These efforts advance the TACF breeding program's goal of stacking resistance to both Phytophthora cinnamomi and Cryphonectria parasitica, ensuring future generations of resilient chestnut trees well adapted to conditions here in the South. Volunteers left with hands-on experience, new knowledge, and a sense of accomplishment, with many eager to apply their skills in their own orchards.

Perkins, M.T., Robinson, A.C., Cipollini, M, and Craddock, J.H.. (2019) Identifying host resistance to Phytophthora cinnamomi in hybrid progeny of Castanea dentata and Castanea mollissima. HortScience 54(2): 221-225. doi.org/10.21273/HORTSCI13657-18
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The 2024 Tennessee Chapter Pollination Workshop was held on June 8 to great success, bringing together 22 enthusiastic volunteers for a day of hands-on learning at the Tennessee Tech Orchard. Established in 2005 using the direct seed planting method, the TTU backcross orchard includes a row of robust, blight resistant F1 hybrids, resulting from a 2004 cross between a naturally occurring Clay County, TN American chestnut, and the Chinese cultivar ‘Gideon.’ These F1s (originally planted as controls in the 2005 backcross orchard), in addition to their blight resistance, exhibit promising resistance to Phytophthora cinnamomi (causal agent of the phytophthora root rot disease, PRR), which has been validated through progeny tests and PRR-screening (Perkins et al., 2019). They were chosen to be the female parents this year in crosses designed to move PRR resistance into the best selections of the southern TACF chapters.

During the 2024 workshop, participants heard a short lecture from Dr. Craddock on the pollination biology of Castanea species and saw an active demonstration of emasculation and bagging of chestnut flowers. They learned about the importance of proper labelling and the labelling conventions of the TACF chapter breeders. They were then engaged directly in hand the pollination techniques used to create hybrid chestnut trees using pollen provided by colleagues in the Georgia, Carolinas, and Alabama chapters ladders and bucket lifts to access the crowns of the trees, successfully performing hundreds of pollinations.
The workshops outcome was exceptional. The volunteers completed 27 distinct crosses. Fruit set was excellent despite the late summer drought. The burs were harvested in September yielding 2,553 hand pollinated seeds! These efforts advance the TACF breeding programs goal of stacking resistance to both Phytophthora cinnamomi and Cryphonectria parasitica, ensuring future generations of resilient chestnut trees well adapted to conditions here in the South. Volunteers left with hands-on experience, new knowledge, and a sense of accomplishment, with many eager to apply their skills in their own orchards.

Perkins, M.T., Robinson, A.C., Cipollini, M, and Craddock, J.H.. (2019) Identifying host resistance to Phytophthora cinnamomi in hybrid progeny of Castanea dentata and Castanea mollissima.  HortScience 54(2): 221-225. https://doi.org/10.21273/HORTSCI13657-18Image attachmentImage attachment+1Image attachment

Join us TODAY, Friday, August 16, 2024, from 11:30AM – 1:00PM (ET), for the next LIVE Chestnut Chat.

Special guests Tony D’Amato and Tim Stout will discuss the role of the American chestnut in forest management strategies that address the impacts of climate change and invasive insects and pathogens on eastern forests. Tony will provide an overview of the frameworks and approaches under evaluation and Tim will bring it to a landowner scale by discussing the strategies being tested and applied on his farm in Shrewsbury, Vermont.

To sign up, visit tacf.org/event/chestnut-chat-adaptive-silviculture-for-climate-change/
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Join us for Chestnut Chat's 50th Episode Spectacular next Friday, July 19th at 11:30AM ET.

Click the link to learn more about or sign up for this webinar. tacf.org/event/chestnut-chat-50th-episode-spectacular/
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