It's time to get growing this week at Meadowview Research Farms! Alongside seeds harvested from Meadowview Research Farms, we’re also planting seeds sourced from partner pollination programs across the American chestnut range. The seeds have been stratified in coolers since being harvested last fall. The process of stratification in coolers mimic natural conditions of moisture and cool temperatures to prepare them for germination. Each seed is carefully placed into its designated pot based on its experiment, covered, and watered in—officially beginning the germination process! Come back and check out the progress soon!
#propagation #seeds #recurrentgenomicselection #americanchestnut #castaneadentata #americanchestnutfoundation #ChestnutResearch #prepping #smallstemassay #meadowviewresearchfarms
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For all you chestnut nerds out there, here's a great read!
From the abstract: Over a century after two introduced pathogens decimated American chestnut populations, breeding programs continue to incorporate resistance from Chinese chestnut to recover self-sustaining populations. Due to complex genetics of chestnut blight resistance, it is challenging to obtain trees with sufficient resistance and competitive growth. We developed high quality reference genomes for Chinese and American chestnut and leveraged large disease phenotype and genotype datasets to develop accurate genomic selection.
View the full abstract and download a PDF of the study here: www.biorxiv.org/content/10.1101/2025.01.30.635736v1.article-info
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Over the past few days, the Meadowview Research Farms team has been hard at work preparing pots to sow seeds for this year's experiments! These seeds play a vital role in Recurrent Genomic Selection, Small Stem Assays, and other trials. With a greenhouse capacity of over 5,000 trees, we’re ready for another year of innovative research. The pot-filling process begins with adding fertilizer to the growing media and filling each pot to the top, ensuring they are ready for sowing. Once all the pots are filled, the tagging process begins. Each pot receives a label indicating its tree ID, genotype, and corresponding experiment. The tagging process is critical as it helps differentiate each pot's journey and helps maintain organization in the greenhouse. Stay tuned for the next post on sowing!
#americanchestnut #castaneadentata #americanchestnutfoundation #ChestnutResearch #prepping #smallstemassay
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Join Lauren as she demonstrates the process of the Generational Passthrough experiment. It starts with the initial inoculation and placement of cryphonectria plugs into young seedlings. She then observes the cankers' development over a two-week period. Next, Lauren extracts the cryphonectria from these cankers, trims and sections the stem into agar for culture and subculture, repeating these steps across ten generations. Finally, she examines the initial and final cultures, where the genome sequence provides crucial insights into the changes of the cryphonectria. Lauren's analysis aims to detect any genotype alterations in the chestnut trees and observe potential evolution in the cryphonectria.
Come back and check out our next post on “Follow the Roots”!
#americanchestnut #castaneadentata #americanchestnutfoundation #ChestnutResearch #ConservationScience #dayinthelife
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At Meadowview Research Farms the chestnut orchards go dormant in the winter, but any seedlings that haven't been planted are allowed to go dormant as well! Potted seedlings are "overwintered" to protect their roots from freezing, ensuring they survive to the next growing season. Trees in the field are insulated from the frigid temperatures by the thermal mass of the soil surrounding their roots, but seedlings do not have the same protection being in small pots. MRF places trees closely together inside of thick vinyl tents to protect potted seedling roots from the low temperatures outside and adds heaters for particularly cold weather. Seedlings for certain research projects also remain dormant indoors in garages that are kept at 40 degrees Fahrenheit throughout the winter. The temperatures have remained consistently low for the past few weeks, but the seedlings are happily waiting out the winter for the next growing season to arrive.
#americanchestnut #castaneadentata #americanchestnutfoundation #ChestnutResearch
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